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1x pbs with azide  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology 1x pbs with azide
    1x Pbs With Azide, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+pbs+with+azide/pm41381473-124-27-31?v=Santa+Cruz+Biotechnology
    Average 93 stars, based on 19 article reviews
    1x pbs with azide - by Bioz Stars, 2026-08
    93/100 stars

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    Bar histograms of mean numbers of CD11b+ leukocytes (a), and total F4/80+ monocytes/macrophages (b), CD3+ T-lymphocytes (c) and CD19+ B-lymphocytes (d) per sciatic nerve section on day 28 post-induction (maximum expected disease severity) are shown. Counts from 4 axial cross-sections per mouse (imaged at 400X magnification) were averaged to obtain a representative count for each mouse studied and mean counts from six mice per experimental group were compared. Statistically significant reductions in CD11b+, F4/80+ and CD3+ leukocytes were observed following CD11b antibody treatment compared to vehicle (PBS)- and isotype antibody-treated controls (a–c), with a significant reduction in CD19+ leukocytes observed with PBS-treated mice only (d). These data imply a robust inhibitory effect on CD11b+ leukocyte infiltration (upregulated in this model based on flow <t>cytometry</t> data), as well as the known effectors of inflammatory demyelination (macrophages and T-lymphocytes) in sm-EAN. Compared to human IVIg-treated mice, CD11b antibody treatment demonstrated statistically significantly reduced total CD11b+ leukocytes and CD3+ T-lymphocytes (a and c) with a 50% reduction in F4/80+ monocytes/macrophages that did not achieve significance (b) probably due to the wide variations in inflammation seen within the same nerve and between nerves of IVIg-treated mice in this model. N=24 axial cross-sections per experimental group. * p <0.05, ** p<0.01 and ns = not significant relative to CD11b antibody treatment.
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    Bar histograms of mean numbers of CD11b+ leukocytes (a), and total F4/80+ monocytes/macrophages (b), CD3+ T-lymphocytes (c) and CD19+ B-lymphocytes (d) per sciatic nerve section on day 28 post-induction (maximum expected disease severity) are shown. Counts from 4 axial cross-sections per mouse (imaged at 400X magnification) were averaged to obtain a representative count for each mouse studied and mean counts from six mice per experimental group were compared. Statistically significant reductions in CD11b+, F4/80+ and CD3+ leukocytes were observed following CD11b antibody treatment compared to vehicle (PBS)- and isotype antibody-treated controls (a–c), with a significant reduction in CD19+ leukocytes observed with PBS-treated mice only (d). These data imply a robust inhibitory effect on CD11b+ leukocyte infiltration (upregulated in this model based on flow cytometry data), as well as the known effectors of inflammatory demyelination (macrophages and T-lymphocytes) in sm-EAN. Compared to human IVIg-treated mice, CD11b antibody treatment demonstrated statistically significantly reduced total CD11b+ leukocytes and CD3+ T-lymphocytes (a and c) with a 50% reduction in F4/80+ monocytes/macrophages that did not achieve significance (b) probably due to the wide variations in inflammation seen within the same nerve and between nerves of IVIg-treated mice in this model. N=24 axial cross-sections per experimental group. * p <0.05, ** p<0.01 and ns = not significant relative to CD11b antibody treatment.

    Journal: Acta neuropathologica

    Article Title: The pathogenic relevance of α M -integrin in Guillain-Barré syndrome

    doi: 10.1007/s00401-016-1599-0

    Figure Lengend Snippet: Bar histograms of mean numbers of CD11b+ leukocytes (a), and total F4/80+ monocytes/macrophages (b), CD3+ T-lymphocytes (c) and CD19+ B-lymphocytes (d) per sciatic nerve section on day 28 post-induction (maximum expected disease severity) are shown. Counts from 4 axial cross-sections per mouse (imaged at 400X magnification) were averaged to obtain a representative count for each mouse studied and mean counts from six mice per experimental group were compared. Statistically significant reductions in CD11b+, F4/80+ and CD3+ leukocytes were observed following CD11b antibody treatment compared to vehicle (PBS)- and isotype antibody-treated controls (a–c), with a significant reduction in CD19+ leukocytes observed with PBS-treated mice only (d). These data imply a robust inhibitory effect on CD11b+ leukocyte infiltration (upregulated in this model based on flow cytometry data), as well as the known effectors of inflammatory demyelination (macrophages and T-lymphocytes) in sm-EAN. Compared to human IVIg-treated mice, CD11b antibody treatment demonstrated statistically significantly reduced total CD11b+ leukocytes and CD3+ T-lymphocytes (a and c) with a 50% reduction in F4/80+ monocytes/macrophages that did not achieve significance (b) probably due to the wide variations in inflammation seen within the same nerve and between nerves of IVIg-treated mice in this model. N=24 axial cross-sections per experimental group. * p <0.05, ** p<0.01 and ns = not significant relative to CD11b antibody treatment.

    Article Snippet: Following a wash, leukocytes were suspended in flow cytometry buffer (10% fetal bovine serum + 0.1% sodium azide in 1X PBS) with added F c block reagent (Becton Dickinson) and incubated on ice for 5 minutes.

    Techniques: Flow Cytometry